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例)研究発表 生命科学科

セミナー第762回生医研セミナー【熊本大学 石黒先生】

  • [開催日時]2018年1月17日(水)16:00-17:00
  • [開催場所]生医研 本館1階 会議室
  • [対象]


演題: A novel nuclear factor plays a crucial role in initiation of meiosis
【Seminar in English】
演者: 石黒 啓一郎 先生
       熊本大学発生医学研究所 染色体制御分野 准教授
       Associate Prof. Kei-ichiro Ishiguro
       Institute of Molecular Embryology and Genetics, Kumamoto University
日時: 2018年1月17日(水) Jan. 17 (Wed), 2018
場所: 病院キャンパス 生体防御医学研究所本館1階 会議室
       Seminar room, Main Building 1F, Medical Institute of Bioregulation
       No.26 building on the following linked map.
The switching from mitotic to meiotic cell cycle is a crucial step for reorganization of meiotic chromosome1, 2 in gametogenesis. In mammals, Stimulated by retinoic acid 8 (STRA8) is required for induction of meiosis.
Although the significance of STRA8 in initiating meiosis is recognized, the underlying mechanism how STRA8 involves in meiotic initiation has been largely elusive. To elucidate the molecular mechanisms of meiotic initiation, we purified STRA8 protein complex from testes of the genetically engineered STRA8 knockin mice. Mass-spectrometric analysis identified novel factors that are associated with STRA8. Notably, StIP1 (Stra8 interacting protein-1), which is encoded by a previously uncharacterized hypothetical gene, shows germ cell specific expression patterns in both male and female germ cells. Immuno-fluorescent analysis shows that StIP1 protein is co-expressed with STRA8 in a limited population of pre-meiotic germ cells in male and female. Importantly, despite of STRA8 expression, initiation of meiosis is abolished in StIP1 KO mice. Remarkably, expression of meiotic prophase genes is largely impaired in the absence of StIP1, accounting for severe arrest of germ cells before meiosis. These results suggest that StIP1 together with STRA8 plays a crucial role in initiation of meiosis.
Here, we discuss our latest unpublished data on the issues of meiotic initiation and the consequent chromosome reorganization.
1. Ishiguro, K. et al. Meiosis-specific cohesin mediates homolog recognition in mouse spermatocytes. Genes & Dev. 28, 594-607 (2014) 2. Kim J, Ishiguro K. et al. Meikin is a conserved regulator of meiosis I specific kinetochore function. Nature 517, 466-471 (2015)