文部科学省科学研究費特定領域研究「性分化機構の解明」 *

研究成果

田中 実 TANAKA, Minoru
基礎生物学研究所 生殖遺伝学研究室
研究項目A01 「性分化の分子基盤の解析」
研究課題 メダカ突然変異体を用いた生殖腺形成過程の細胞系譜・相互作用解析(平成17年度〜平成18年度)
     突然変異体と三次元細胞系譜解析とを用いた性分化過程細胞相互作用解析(平成19年度〜平成20年度)

2009年発表論文

Herpin, A., Nakamura, S., Wagner, T., Tanaka, M. and Schartl, M. A highly conserved cis-regulatory motif directs differential gonadal synexpression of Dmrt1 transcripts during gonad development. Nucleic Acids Res. 2009, in press.

Aoki, Y., Nakamura, S., Ishikawa, Y. and Tanaka, M. Expresssion and sytenic analysis of four nanos genes in medaka Zool. Sci. 26., 112-118, 2009

2008年発表論文

Sasado, T., Yasuoka, A., Abe, K., Mitani, H., Furutani-Seiki, M., Tanaka, M. and Kondoh, H. Distinct contributions of CXCR4b and CXCR7/RDC1 receptor systems in regulation of PGC migration revealed by medaka mutants kazura and yanagi. Dev. Biol. 320, 328-339, 2008

Nakamura, S., Saito, D. and Tanaka, M. Generation of transgenic medaka using modified bacterial artificial chromosome. Dev. Growth Differ. 50, 415-417, 2008

Nakabayashi, T., Nagao, I., Kinjo, M., Aoki, Y., Tanaka, M. and Ohta, N. Stress-induced environmental changes in a single cell as revealed by fluorescence lifetime imaging. Photonchem. Photobiol. Sci. 7, 671-674, 2008

Tanaka, M., Saito, D., Morinaga, C. and Kurokawa, H. Cross talk between germ cells and gonadal somatic cells is critical for sex differentiation of the gonads in the teleost fish, medaka (Oryzias latipes). Dev. Growth Differ. 50, 273-278, 2008 (Review Article)

Aoki, Y., Nagao, I., Saito, D., Ebe, Y., Kinjo, M. and Tanaka, M. Temporal and spatial localization of three germline-specific proteins in medaka. Dev. Dyn. 273, 800-807, 2008

Nakamura, S., Aoki, Y., Saito, D., Kuroki, Y., Fujiyama, A., Naruse, K. and Tanaka, M. Sox9b/sox9a2-EGFP transgenic medaka reveals the morphological reorganization of the gonads and a common presursor of both the female and male supporting cells. Mol. Reprod. Dev. 75, 472-476, 2008

Nagao, I., Aoki, Y., Tanaka, M. and Kinjo, M. Analysis of the molecular dynamics of medaka nuage proteins by fluorescence correlation spectroscopy and fluorescence recovery after photobleaching. FEBS J. 275, 341?349, 2008

2007年発表論文

Kurokawa, H., Saito, D., Nakamura, S., Katoh-Fukui, Y., Ohta, K., Aoki, Y., Baba, T., Morohashi, K. and Tanaka, M. Germ cells are essential for sexual dimorphism in the medaka gonad. Proc. Acad. Natl. Sci. USA 104, 16958-16963, 2007 (Direct Submission to PNAS Office)

Saito, D., Morinaga, C., Aoki, Y., Nakamura, S., Mitani, H., Furutani-Seiki, M., Kondoh, H. and Tanaka, M. Proliferation of germ cells during gonadal sex differentiation in medaka: insights from germ cell depleted mutant zenzai. Dev. Biol. 310, 280-290, 2007

Morinaga, C., Saito, D., Nakamura, S., Sasaki, T., Asakawa, S., Shimizu, N., Mitani, H., Furutani-Seiki, M., Tanaka, M (Corresponding Author), and Kondoh, H. The hotei mutation of medaka in the anti-Mullerian hormone receptor causes the dysregulation of germ cell and sexual development. Proc. Acad. Natl. Sci. USA 104, 9691-9696, 2007 (Direct Submission to PNAS Office)

Takamatsu, N., Kurosawa, G., Takahashi, M., Inokuma, R., Tanaka, M., Kanamori, A. and Hori, H. Duplicated Abd-B class genes in medaka hoxAa and hoxAb clusters exhibit different expression patterns in pectoral fin buds Dev. Genes Evol. 217, 263-273, 2007

Hano, T., Oshima, Y., Kinoshita, M., Tanaka, M., Mishima, N., Ohyama, T., Yanagawa, T., Wakamatsu, Y., Ozato, K. and Honjo, T. Quantitative bioimaging analysis of gonads in olvas-GFP/ST-II YI medaka (transgenic Oryzias latipes) exposed to ethinylestradiol, Environ. Sci. Tech. 41, 1473-1479, 2007

2006年発表論文

Shuhei Nakamura, Daisuke Kobayashi, Yumiko Aoki, Hayato Yokoi, Youko Ebe, Joachim Wittbrodt and Minoru Tanaka. Identification and lineage tracing of two population of somatic gonadal precursors in medaka embryos. Dev. Biol. 295, 678-688, 2006

Hiromi Kurokawa, Yumiko Aoki, Shuhei Nakamura, Youko Ebe, Daisuke Kobayashi and Minoru Tanaka. Time-lapse analysis reveals different modes of primordial germ cell migration in the medaka Oryzias latipes. Develop. Growth Differ. 48, 209-221. 2006

Saito, T., Fujimoto, T., Maekawa, S., Inoue, K., Tanaka, M., Arai, K. and Yamaha, E. Visualization of primordial germ cells in vivo using GFP-nos1 3'UTR mRNA. Int. J. Dev. Biol. 50, 691-700, 2006

2005年発表論文

Takeshi Hano, Yuji Oshima, Toshiaki, Oe, Masato Kinoshita, Minoru Tanaka, Yuko Wakamatsu, Kenjiro Ozato and Tsuneo Honjo. Quantitiative Bio-imaging analysis for evaluation of sexual differentitation in germ cells of olvas-GFP/STII YI medaka (Oryzias latipes) nanoinjected in ovo with ethinylestradiol. Environmental Toxicology and Chemistry 24, 70-77, 2005